FastQCFastQC Report
Wed 16 Nov 2016
SRR1945435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30045572
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG108500.017.3290735
TATACTG156800.016.4449375
TCTATAC197650.014.6331223
CCGTGAG75550.014.5382859
CATACAC919350.014.4450273
ATACCGT96250.014.3678226
TACCGTG80400.014.1944087
ACATGAC666600.014.1466168
CTATACT163650.013.9959414
ACACATG854650.013.9396846
ACCGTGA96500.013.8740548
CTAGTAC102200.013.6225613
TAGGCTG90350.013.526655
GTGTATG809050.013.5082671
GTCGCGC16300.013.46071158
CATGACA693900.013.4577959
CACATGA800450.013.3236447
TATGAGC122950.013.2767494
TCATACA986800.013.2279362
TATACAG149500.012.8161735
Produced by FastQC (version 0.11.5)