FastQCFastQC Report
Wed 16 Nov 2016
SRR1945493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56395814
Sequences flagged as poor quality0
Sequence length40-101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG301300.09.5586854
GGTCGCC66300.09.00240144-45
AGAGCGT159250.08.8522429
GATCGGA398700.07.64018252
CGGAAGA399800.07.2966345
AAGAGCG203000.07.1278028
GCACACG104950.07.07068112-13
ATCGGAA433200.07.0665263
CGCCGTA81650.07.03141546-47
GAGCGTC101750.06.88157110-11
TGCCGTC79650.06.686468648-49
TGGTCGC88800.06.686264542-43
AGCGTCG106550.06.637058310-11
TATGCCG83000.06.32415846-47
GCGTCGT114750.06.162740712-13
GAAGAGC498750.06.0821347
GCCGTCT87450.06.043483350-51
ATGCCGT86750.05.922858748-49
AGAGCAC261950.05.87894969
CGTATGC104300.05.4756744-45