FastQCFastQC Report
Wed 16 Nov 2016
SRR1945591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40960618
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT4337161.0588609771463897Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT2670260.6519091093791602Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGG644040.15723395579627242Illumina Paired End PCR Primer 2 (97% over 49bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGG474730.11589913023284952Illumina Paired End PCR Primer 2 (97% over 49bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG425130.10378993793501846Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT2010950.059.643959
GCGGTTC1966500.059.2875611
AGCGGTT1982600.059.18894610
AGAGCGG2106200.057.9355628
TATGCCG1495100.057.42554543
CGTATGC1515450.057.1606541
GTTCAGC2008950.056.82942214
ATGCCGT1262550.056.82495544
GTATGCC1522100.056.7899442
TGCCGTC1238550.056.60396245
GGTTCAG2050700.056.3363313
GAGACCG1806600.056.2784730
CGAGACC1807850.056.26914629
CGGTTCA2063150.056.2436212
GCCGTCT1143500.056.11120246
TCGTATG1600100.056.0490940
AATGCCG1810550.055.8896424
CAGCAGG1993650.055.60813517
AGACCGA1861550.055.33516731
CCGATAT1073200.055.15080634