FastQCFastQC Report
Wed 16 Nov 2016
SRR1945659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67512456
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT5177310.7668673762957164Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGG1266320.18756835035004502Illumina Paired End PCR Primer 2 (97% over 49bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1259070.1864944744418719Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG809550.11991120571883801Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1279750.056.7768943
GAGACCG3229050.056.03851730
CGAGACC3253500.055.9474729
CGTATGC1360050.055.9175141
GTATGCC1337650.055.90723842
GACCGAT2971500.055.72829432
AGACCGA3216850.055.5224931
CCGATAT2023400.055.32439834
AATGCCG3356800.055.0112724
TGCCGTC963550.054.68639845
ATGCCGT1015850.054.67211544
GCGGTTC3788100.054.3771711
ACCGATA2127950.054.15286333
TGCCGAG3427200.054.1007926
TCGTATG1679000.054.0522140
CGATATC2047650.054.04123735
GAATGCC3466150.053.8835423
AGCGGTT3849400.053.85216510
TATCGTA1384150.053.8389238
CCGAGAC3402000.053.74117328