FastQCFastQC Report
Wed 16 Nov 2016
SRR1945623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54457934
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT10911562.003667638217785Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT4148770.7618302229386814Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGG1221530.22430707709183384Illumina Paired End PCR Primer 2 (97% over 49bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG915220.16805999287450016Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGC748090.13737024985193158Illumina Paired End PCR Primer 2 (97% over 49bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGG570240.10471201496553284Illumina Paired End PCR Primer 2 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT3489050.059.7088859
AGCGGTT3455200.059.60183710
GCGGTTC3440450.059.57300611
AGAGCGG3667850.058.0576748
TATGCCG2344600.057.3885243
CGTATGC2403900.057.05944441
ATGCCGT2025050.056.88586444
GTATGCC2393400.056.8527142
TGCCGTC1951700.056.7893545
GTTCAGC3546900.056.71686614
CGGTTCA3602050.056.56020412
GCCGTCT1816000.056.455246
GGTTCAG3603950.056.36004613
CAGCAGG3520800.056.02594817
AATGCCG3344250.056.00414724
GAGACCG3227600.055.85698330
CGAGACC3257350.055.84715729
TCGTATG2602150.055.83853540
CAGGAAT3461500.055.44946720
CCGATAT2175250.055.4085534