FastQCFastQC Report
Mon 14 Nov 2016
SRR1946529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1946529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53589896
Sequences flagged as poor quality0
Sequence length32-64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG93150.022.50446535
GCGTCGT97500.020.96688731
GAGTGTA117200.020.51187947
GTAGGGA110900.019.94408438
AGTGTAG125800.019.7887848
GTCGTGT104550.019.68757633
TGTAGGG111650.019.68136237
CGGCATT92500.018.2561021
GTGTAGG119150.018.24537336
TCGTGTA116300.018.24300434
CGTCGTG113750.018.04036332
GTGTAGA139600.017.30647749
GTAGATC105650.017.18680851
TAGGGAA143800.015.53504239
AGAGTGT153550.015.43508246
GCATTCC117750.014.4538983
GGCATTC118050.014.3830182
CCTGCTG119650.013.8702368
GCTGAAC120250.013.80048811
TGTAGAT171100.013.71977850