FastQCFastQC Report
Mon 14 Nov 2016
SRR1946470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1946470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26018518
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG89600.012.6931295
CTATACT138600.012.4102114
TCTATAC166050.012.3115213
ACATGAC591050.011.7204358
CATGACA595600.011.4101579
CATACAC766850.011.3927953
TATACTG134750.011.2302245
GTATAGG99100.011.2008331
TACCGTG74400.011.180647
ACACATG783550.011.1561036
GAGCGCG18600.011.1174737
CACATGA764800.010.9011447
GTATATA360650.010.8765741
GTATAGA183950.010.8412171
ATACCGT79600.010.7454526
TATACAG112800.010.5824425
CCTAGAC58400.010.3809763
TCATACA853900.010.2754172
GTATTAG160500.010.2273331
ATCATAC845650.09.9390731
Produced by FastQC (version 0.11.5)