FastQCFastQC Report
Tue 15 Nov 2016
SRR1946270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1946270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19946584
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC7150.017.7545998
TATACCG64700.013.7394075
TCGCGCG10050.012.6315399
TCTATAC122900.011.2124643
TATACTG97700.011.1687315
CTATACT97950.010.7079814
ACTGTGC27250.010.6974028
ATACCGT57650.010.4394336
CATACAC551850.010.30363753
GTATAGA126900.010.0862241
TATACAG85000.010.015445
TACCGTG52150.010.007847
CTAGGCT47550.09.8913754
ACACATG548450.09.8588676
TAGGCTG59300.09.835075
GCTGTCC49450.09.7931888
CCGTGAG41800.09.6734369
ACATGAC453150.09.43137558
CCCTATA70750.09.3056922
GTCTAGG40650.09.2608481
Produced by FastQC (version 0.11.5)